Included with the BLAT source code is a useful utility that converts FASTA references to 2bit format. The utility, faToTwoBit, works simply by providing the original reference .fa file to be converted to a .2bit file. Here is the syntax to run the program:
A few simple options are also included:
For more details on these options, type faToTwoBit at the command line. Hope this 2bit reference database speeds up your BLAT searches!
Welcome to the Genome Toolbox! I am glad you navigated to the blog and hope you find the contents useful and insightful for your genomic needs. If you find any of the entries particularly helpful, be sure to click the +1 button on the bottom of the post and share with your colleagues. Your input is encouraged, so if you have comments or are aware of more efficient tools not included in a post, I would love to hear from you. Enjoy your time browsing through the Toolbox.