Included with the BLAT source code is a useful utility that converts FASTA references to 2bit format. The utility, faToTwoBit, works simply by providing the original reference .fa file to be converted to a .2bit file. Here is the syntax to run the program:
A few simple options are also included:
-noMask
-stripVersion
-ignoreDups
For more details on these options, type faToTwoBit at the command line. Hope this 2bit reference database speeds up your BLAT searches!
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